Original research articles

Evaluation of the settling of malolactic starters within the indigenous microflora of wines


Six malolactic starters were experimented. The objective was an evaluation of the capacity of each one to settle in different wines. First we obtained the genomie DNA fingerprinting with Not I a rare cutting enzyme after pulse field gel electrophoresis (PFGE). Previous results with Leuconostoc oenos strains of our collection showed that this enzyme was the most appropriated for identification at the strain level. However, we did not obtain six different patterns, three starters showed the same. Therefore Apa I and Sfi I patterns were also established. Contrary to the expected results A, B and C finally had the same fingerprinting with the three enzymes. Another investigation was conduced using the Southem hybridization of EcoRI restricted DNA with the φMC10 prophage DNA as probe. Our again all three starters showed the same pattern. They probably were prepared with the same strain. Wines in vat or barrels were inoculated just after racking. Malolactic fermentation had already begun in some of them before inoculation. However malic acid was degraded more quickly in all the inoculated wines than in the controls. The PFGE patterns were obtained from bacteria isolated from wines at the end of malolactic fermentation. In 15 cases of 21, the pattern of the starter appeared alone or superimposed with other bacteria. This proved that, even in the worst conditions of this experimentation, where the indigenous microflora was very abundant and active malolactic starters could survive and predominate. The influence of these starters on the sensorial quality of wines needs to be studied.


Emmanuel Gindreau


Affiliation : UMR OEnologie - Ampélologie INRA -Université Victor Segalen Bordeaux 2, 351 cours de la libération, 33405 Talence, France; SARCO, ZA La Jacquotte, Rue Aristide Bergès, 33270 Floirac, France

Annick Joyeux

Affiliation : Faculté d'OEnologie, Unité associée INRA/Université Victor Segalen Bordeaux II, 351, cours de la Libération, 33405 Talence (France)

Gilles de Revel

Affiliation : Univ. de Bordeaux, ISVV, EA 4577, Unité de recherche Œnologie, 33882 Villenave d'Ornon, France; INRA, ISVV, USC 1366 Œnologie, 33882 Villenave d'Ornon, France

Olivier Claisse

Affiliation : UMR OEnologie - Ampélologie INRA -Université Victor Segalen Bordeaux 2, 351 cours de la libération, 33405 Talence, France

Aline Lonvaud-Funel

Affiliation : UMR 1219 OEnologie, INRA - Université de Bordeaux, ISVV, 210, chemin de Leysotte, CS 50008, 33882 Villenave d'Ornon, France


No supporting information for this article

Article statistics

Views: 447


PDF: 161