Original research articles

Genetic determination of Saccharomyces cerevisiae et Saccharomyces bayanus species by PCR/RFLP analysis of the MET2 gene


Several yeast strains of the species S. cerevisiae, S. bayanus and S. paradoxus, first identified by hybridization experiments and DNA/DNA hybridization were characterized by using Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (PCR/RFLP) of the MET2 gene. The concordance between this tool and classical genetic analyses did not reveal any exception for all the strains analysed, so PCR/RFLP of the MET2 gene proves to be a reliable and fast tool for delimiting S. cerevisiae and S. bayanus. OEnological strains race bayanus, chevalieri, capensis gave S. cerevisiae restriction patterns, whereas most of strains race S. uvarum belong to S. bayanus and displayed a specific chromosomal band patterns different from band patterns of S. cerevisiae strains. To avoid confusion in oenological terminology, oenologists should no longer use the name of bayanus to designate industrial or wild S. cerevisiae Gal- strains, and should consider S. bayanus as a distinct species.


Isabelle Masneuf-Pomarède


Affiliation : Université de Bordeaux, Unité de Recherche Œnologie EA 4577, USC 1366 INRA, ISVV, F-33140 Villenave d’Ornon, France; Bordeaux Sciences Agro, F-33175 Gradignan, France

Michel Aigle

Affiliation : Institut de Biochimie et de Génétique Cellulaire, 1, rue Camille Saint-Saëns, 33077 Bordeaux (France)

Denis Dubourdieu

Affiliation : Faculté d’oenologie, UMR 1219 OEnologie, Université de Bordeaux, ISVV, 210, chemin de Leysotte, CS 50008, 33882 Villenave d'Ornon, France


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