Original research articles

Application of a reversed-phase HPLC method for quantitative p-coumaric acid analysis in wine

Abstract

Aims: This paper presents a rapid chromatographic method to monitor the concentration of p-coumaric acid in wine and in bioconversion studies.

Methods and results: RP-HPLC method was validated in synthetic wine medium and in natural red wine. Mobile phase composition was water 77%, acetonitrile 23%. Formic acid was added to control pH at 3.5. The flow was 0.7 mL/min and the temperature 30 °C. The detection was done using UV at 305 nm. The linearity range was validated between 0.5 and 15 mg/L. The resolution was respectively 5.35 and 2.99. The detection and quantification limits were 0.01 mg/L and 0.04 mg/L. This method was used to study p-coumaric acid bioconversion into 4-ethylphenol and 4-vinylphenol, and to study this acid adsorption in enological conditions.

Conclusions: This paper presented a simple HPLC method to monitor the concentration of p-coumaric acid in synthetic media and natural wine. It was used to study the p-coumaric acid bioconversion rates and mechanism.

Significance and impact of the study: This method is useful to monitor p-coumaric acid concentration, which helps to predict amounts of 4-ethylphenol or 4-vinylphenol that can be produced in wine. This method can be helpful to control undesirable phenolic flavors potential in wine.

Authors


Dominique Salameh

dominique.salameh@fs.usj.edu.lb

Affiliation : Laboratoire de Génie Chimique (UMR CNRS 5503), 4, allée Emile Monso, 31432 Toulouse, France; Département de Chimie, Université Saint-Joseph, Faculté des Sciences, Mar Roukoz, Mkallès Po.Box 11514, 1107-2050, Beirut, Lebanon


Cédric Brandam

Affiliation : Laboratoire de Génie Chimique (UMR CNRS 5503), 4, allée Emile Monso, 31432 Toulouse, France


Toufic Rizk

Affiliation : Département de Chimie, Université Saint-Joseph, Faculté des Sciences, Mar Roukoz, Mkallès Po.Box 11514, 1107-2050, Beirut, Lebanon


Roger Lteif

Affiliation : Département de Chimie, Université Saint-Joseph, Faculté des Sciences, Mar Roukoz, Mkallès Po.Box 11514, 1107-2050, Beirut, Lebanon


Pierre Strehaiano

Affiliation : Laboratoire de Génie Chimique (UMR CNRS 5503), 4, allée Emile Monso, 31432 Toulouse, France

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