Original research articles

Use of image analysis for the study of phenolic compounds of the grape berry skin (Vitis vinifera L., cv Cabernet franc)

Abstract

The localization and quantitative determination of phenolics in grape berry skins, from the onset of veraison, constitute the first step to understand the évolution of these compounds throughout the maturation process. Histological techniques are appropriate to study the evolution of phenolics but manual countings are long and drudgery and do not allow for reliable quantitative results. The image analysis software "Scion Image" proved to be a good tool to improve the quantitative results. This method permitted also to measure the cells area and the area occupied by phenolic compounds inside the vacuoles. Image analysis could be helpful to the understanding of the évolution of phenolics during maturation and possibly contribute to explain their extraction during macération.

Authors


Michel Chevalier

Affiliation : UMR 1259, Génétique et Horticulture (GenHort), INRA Angers-Nantes, 42 rue Georges Morel, BP 60057, 49071 Beaucouzé Cedex, France


Emilie Perrochon

Affiliation : INRA, Unité expérimentale Vigne et Vin, 49071 Beaucouzé cedex, France


A. Clement

Affiliation : Laboratoire d'Ingénierie des Systèmes Automatisés. Institut universitaire de Technologie, dép. G.E.I.I. 4, bd Lavoisier B.P. 42018, 49016 Angers cedex France


Anne-Laure Dubot

Affiliation : Institut National de la Recherche Agronomique, Unité expérimentale Vigne et Vin, 49071 Beaucouzé cedex


Marie Tellier

Affiliation : INRA, Unité d'amélioration des espèces fruitières et ornementales, 49071 Beaucouzé cedex, France


Yves Cadot

Affiliation : Institut de Recherche en Horticulture et Semences UMR1345 (INRA / Agrocampus-Ouest / Université d'Angers), 42 rue Georges Morel BP 60057, 49071 Beaucouzé Cedex, France


Gérard Barbeau

Affiliation : INRA, UE1117 UVV, F-49071 Angers, France

cvlr@angers.inra.fr

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