Original research articles

Genetic characterization of Albanian grapevine cultivars by microsatellite markers

Abstract

A recently restored ampelographic collection of Albanian grapevine accessions has been submitted to genetic profiling with eleven nuclear microsatellite markers, widely used in other studies. Microsatellite profiling resulted in 28 single profiles for 29 accessions. Two cultivars, Shesh I bardhë and Pucalla, were found to be synonyms. Genetic profiles of Albanian cultivars were compared at 8 microsatellite loci to 29 most commonly cultivated Greek cultivars. Albanian cultivars were found to be more closely related to Greek cultivars from Peloponnese. One Greek cultivar named Dempina was found to be genetically close to two Albanian cultivars Debina teki and Debina kala, which are homonyms Another cultivar, known as Toska or Sinambel displayed a tri-allelic profile at 5 loci over 10 analysed loci. Such a high number of tri-allelic loci found in one individual favours the hypothesis of triploidy but the chimerism hypothesis cannot be excluded without further work.

Authors


Emmanuel D. Ladoukakis

Affiliation : Laboratory of Plant Physiology and Biotechnology, Departement of Biology, University of Crete, Vassilika Vouton, PO BOX 2208, 71409 Heraklion, Greece; Centre for the Study of Evolution & School of Life Sciences, University of Sussex, BN1 9QG Brighton, UK

e.ladoukakis@sussex.ac.uk

François Lefort

Affiliation : Laboratory of Plant Physiology and Biotechnology, Departement of Biology, University of Crete, Vassilika Vouton, PO BOX 2208, 71409 Heraklion, Greece; Applied Genetics Laboratory, School of Engineering of Lullier, University of Applied Sciences of Western Switzerland, 150 route de Presinge, 1254 Jussy, Switzerland


Petraq Sotiri

Affiliation : Institute of Biological Research, The Academy of Sciences of Albania, Rruga Sami Frasheri Nr.5 Tirana, Albania


Arjola Bacu

Affiliation : Institute of Biological Research, The Academy of Sciences of Albania, Rruga Sami Frasheri Nr.5 Tirana, Albania


Efigjeni Kongjika

Affiliation : Institute of Biological Research, The Academy of Sciences of Albania, Rruga Sami Frasheri Nr.5 Tirana, Albania


Kalliopi A. Roubelakis-Angelakis

Affiliation : Laboratory of Plant Physiology and Biotechnology, Departement of Biology, University of Crete, Vassilika Vouton, PO BOX 2208, 71409 Heraklion, Greece

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