Original research articles

Molecular profiling of Vitis vinifera Chardonnay obtained by somatic embryogenesis

Abstract

With the help of microsatellite profiling, we showed that Vitis vinifera Chardonnay clone 96 is a periclinal chimera plant which is composed at least of two distinct cell layers. Performing somatic embryogenesis allowed us to separate the two cell layers and to regenerate L1 plants. These regenerated L1 plants did not show phenotypic differences to the parental clone when grown in greenhouse conditions, suggesting therefore that the phenotype of Chardonnay 96 did not result of an interaction between the two distinct cell layers L1 and L2.

Authors


Christophe Bertsch

Affiliation : Laboratoire Biotechnologies Vigne et Environnement, Université de Haute-Alsace, 29 rue de Herrlisheim, 68008 Colmar, France


Flore Kieffer

Affiliation : Laboratoire Biotechnologies Vigne et Environnement, Université de Haute-Alsace, 29 rue de Herrlisheim, 68008 Colmar, France


Cécile Triouleyre

Affiliation : Laboratoire Biotechnologies Vigne et Environnement, Université de Haute-Alsace, 29 rue de Herrlisheim, 68008 Colmar, France


Gisèle Butterlin

Affiliation : INRA, UMR 1131, Vigne et Vin d’Alsace, 28, rue de Herrlisheim 68000 Colmar, France


Didier Merdinoglu

Affiliation : UMR ULP-INRA 1131, Vigne et Vins d'Alsace, B.P. 507, 28 rue de Herrlisheim, 68000 Colmar, France


Bernard Walter

Affiliation : Laboratoire Biotechnologies Vigne et Environnement, Université de Haute-Alsace, 29 rue de Herrlisheim, 68008 Colmar, France

b.walter@uha.fr

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