Original research articles

Practical application of immunofluorescence for the detection of bacterial contaminants during vinification

Abstract

This study presents a rapid and specific microscopie technique for detecting and identifying populations of lactic acid bacteria in musts, wines, and inoculum starter cultures. Through the use of fluorescent antibodies, this procedure can be performed in less than two hours, and it is effective with Leuconostoc, Pediococcus and Lactobacillus concentrations as small as 102 cells/ml. Implementation of this technique will assist winemakers in controlling malolactic fermentations and in preventing lactic acid bacterial spoilage.

Authors


Marielle Bouix

Affiliation : Département de Microbiologie Industrielle, Ecole Nationale Supérieure des Industries Alimentaires, 1 avenue des Olympiades, 91744 Massy cedex (France)


Claire Busson

Affiliation : Ecole Nationale Supérieure des Industries Agricoles et Alimentaires, 1 avenue des Olympiades, 91305 Massy (France)


Monique Charpentier

Affiliation : Moët et Chandon, 6 rue Croix de Bussy, 51200 Epernay (France)

mcharpen@moeum.fr

Jean-Yves Leveau

Affiliation : Département de Microbiologie Industrielle, Ecole Nationale Supérieure des Industries Alimentaires, 1 avenue des Olympiades, 91744 Massy cedex (France)


Bruno Duteurtre

Affiliation : Laboratoire de Recherches, Champagne Moët & Chandon, 6, rue Croix de Bussy, 51504 Épernay, France

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