Original research articles

Comparison of pectolytic and proteolytic activities of six clarifying commercial preparations. Incidence of secondary enzymatic activities on proteins in Champagne musts


In order ta estimate c1arifying aptitude and the effects of prefermentation treatment by commercial pectinases, polygalacturonase (PG), pectin Iyase (PL), pectinmethylesterase (PME) and proteolytic activities of six pectinases samples were studied. PG activity is preponderate and proportional to the relative protein concentration.

These one, submitted to both SDS-PAGE and isoelectric focusing technics, show molecular weights ranging from 20 ta 100 kDa and isoelectric points ranging from 3.00 ta 7.40. Total protein of Champagne grape varieties Chardonnay (CH) and Pinot noir (PN) - were submitted to an isoelectric focusing. All of the pI's ranging from 3.30 to 4.55 (CH) and from 3.30 to 4.90 (PN). Proteases are put in evidence in commercial pectinases using fluorescamine- casein as substrate. However, they seem to have no incidence upon must proteins.


Laurent Berthier

Affiliation : UFR des Sciences Exactes et Naturelles, Laboratoire d'OEnologie, Moulin de la Housse, B.P. 1039,51687 Reims cedex 2 (France)

Richard Marchal


Affiliation : Laboratoire d'oenologie, Université de Reims, URVVC, UPRES EA 2069 B.P. 1039, 51687 Reims cedex, France

Alain Maujean

Affiliation : Laboratoire d'OEnologie - U.F.R. Sciences - Université de Reims, B.R 1039 - 51687 Reims cedex 02 (France)


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