Leuconostoc oenos strains: m, L2 ST, X2L, have been isolated from different Argentinian red wines. Amino acids, vitamins and base requirements were determined in a synthetic medium containing L-malic acid, citric acid or both organic acids. The organisms differed in their requirements. The growth of the four L. oenos strains studied was greater in the synthetic medium with L-malic acid plus citric acid. In this medium, L. oenos m, L2, and ST eliminated their amino acid requirements, however X2L in the same medium increased these requirements. In respect of the vitamin and base requirements, L. oenos m and L2 showed no significant difference by the addition of L-malic acid, citric acid or both organic acids to the synthetic medium. L. oenos ST needed L-malic acid plus citric acid to avoid these requirements, while the X2L strain increased its requirements with both organic acids.
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