The effects of polyamines were examined for growth and polyamine contents in cultings, callus and primary adventitious roots of Vitis vinifera L. Variations in free, conjugated and wall-bound polyamines in cuttings were observed during rhizogenesis. The main polyamines in cuttings were conjugated polyamines while in callus and primary adventitious roots they were free polyamines. Exogenous polyamine addition did not modify the total number of roots per cutting but increased the mean size and number of long roots. Moreover, exogenous polyamines increased polyamine levels in callus and roots, particurlarly wall-bound and conjugated polyamines. The involvement of these classes of polyamines in morphogenic processes is discussed.
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