Original research articles

Mise au point d'une mesure rapide de l'activité laccase dans les moûts et dans les vins par la méthode a la syringaldazine. Application à l'appréciation de l'état sanitaire des vendanges

Abstract

The syringaldazine assay has been adapted for quantitative determination of laccase activity from Botrytis cinerea, in must and new wines. This colorimetric method is fast, specifie and sensible. It allows to appreciate the level of contamination of the crops by Botrytis cinerea.

The assay can be performed in presence of tyrosinase but the phenolic compounds of the grape must be discarded because of their inhibitor effects on syringaldazine oxidation by laccase.

So measured in botrytised grapes juices, laccase activity is related with the stages of development of the fungus.

Authors


Denis Dubourdieu

Affiliation : Faculté d’oenologie, UMR 1219 OEnologie, Université de Bordeaux, ISVV, 210, chemin de Leysotte, CS 50008, 33882 Villenave d'Ornon, France

editorialsecretary@oeno-one.eu

Catherine Grassin

Affiliation : Institut d'OEnologie, Université de Bordeaux II, 351, cours de la Libération, 33405 Talence Cedex (France)


Catherine Deruche

Affiliation : Institut d'CEnoiogie, Université de Bordeaux II, 351, cours de la Libération, 33405 Talence Cédex (France)


Pascal Ribéreau-Gayon

Affiliation : Faculté d’OEnologie, Université Victor Segalen Bordeaux 2, 351 cours de la Libération, 33405 Talence cedex (France)

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