Original research articles

Use of SSR markers to identify grapevine cultivars registered in the Czech Republic

Abstract

In this study we analyzed 51 grapevine cultivars registered in the Czech Republic using six highly informative SSR loci, and determined SSR profiles typical for each cultivar. All cultivars were easily distinguished on the basis of SSR allele length, except for berry color mutants (Pinot and Chasselas group). The average number of detected alleles per locus was 13.2, while observed heterozygosity is between 0.745 (VrZAG 79) and 0.922 (VVMD 7). The lengths of the alleles were compared with results from other laboratories; lengths were either the same or different by 1-3 bp. We also tested a new approach to microsatellite data comparison using a coding system based on reference alleles as a size standard (THIS et al., 2004). The obtained profiles were mostly in accordance with profiles of reference cultivars (THIS et al., 2004); we discuss the possible reasons for observed discrepancies.

A third allele in the microsatellite spectrum was observed in five samples where template DNA originated from leaves. Additional analyses with DNA isolated from phloem-enriched samples (L2 meristem layer) were performed and typical diallelic profiles obtained. The reason for the detected triallelism is assumed to be a periclinal chimerism.

Authors


Katherina Moravcova

xmoravc2@node.mendelu.cz

Affiliation : Mendeleum Institute, Horticultural Faculty in Lednice, Mendel University of Agriculture and Forestry in Brno, Zahradnická fakulta MZLU, Valtická 334, 691 44 Lednice, Czech Republice


Miroslav Baranek

Affiliation : Mendeleum Institute, Horticultural Faculty in Lednice, Mendel University of Agriculture and Forestry in Brno, Zahradnická fakulta MZLU, Valtická 334, 691 44 Lednice, Czech Republice


Miroslav Pidra

Affiliation : Mendeleum Institute, Horticultural Faculty in Lednice, Mendel University of Agriculture and Forestry in Brno, Zahradnická fakulta MZLU, Valtická 334, 691 44 Lednice, Czech Republice

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