Original research articles

Nitrogen metabolism in a grapevine in vitro system

Abstract

Ammonium, nitrate, nitrite, protein and individual and total amino acid contents were determined in grapevine (cv Sauvignon) cultured in vitro. The enzyme activities of nitrate and nitrite reductases, glutamine synthetase, glutamate synthetase and dehydrogenase were also determined. The nitrogen taken up by the plants was 70% of the total nitrogen in the medium after 75 days of in vitro culture. Most of the nitrogen taken up was recovered in the leaves, yet only ammonia and amino acid concentrations were significantly higher in leaves. In roots, glutamine was the most abundant amino acid. In leaves, the most abundant amino acids were aspartate, glutamate, glutamine, alanine, arginine and g-aminobutirate. All enzyme activities were higher in roots than in leaves. These results suggest that both roots and leaves incorporate inorganic nitrogen into organic forms.

Authors


Nuria Llorens

Affiliation : Unitat d'Enologia-Centre de Referència en Tecnologia dels Aliments (UE-CeRTA), Departament de Bioquímica i Biotecnologia, Facultat d'Enologia de Tarragona, Universitat Rovira i Virgili, Ramón y Cajal, 70, 43005 Tarragona, Spain


Lluís Arola

Affiliation : Unitat d'Enologia-Centre de Referència en Tecnologia dels Aliments (UE-CeRTA), Departament de Bioquímica i Biotecnologia, Facultat d'Enologia de Tarragona, Universitat Rovira i Virgili, Ramón y Cajal, 70, 43005 Tarragona, Spain


Cinta Bladé

Affiliation : Unitat d'Enologia-Centre de Referència en Tecnologia dels Aliments (UE-CeRTA), Departament de Bioquímica i Biotecnologia, Facultat d'Enologia de Tarragona, Universitat Rovira i Virgili, Ramón y Cajal, 70, 43005 Tarragona, Spain


Albert Mas

Affiliation : Unitat d'Enologia-Centre de Referència en Tecnologia dels Aliments (UE-CeRTA), Departament de Bioquímica i Biotecnologia, Facultat d'Enologia de Tarragona, Universitat Rovira i Virgili, Ramón y Cajal, 70, 43005 Tarragona, Spain

amb@fe.urv.es

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