Original research articles

Transient expression of uidA gene in grapevine protoplasts after PEG-mediated transformation

Rahma Jardak, Ahmed Mliki, Abdelwahed Ghorbel, Götz M. Reustle
Received : 19 September 2001; Accepted : 25 February 2002; Published : 30 June 2002
DOI: https://doi.org/10.20870/oeno-one.2002.36.2.975

Abstract

Leaf protoplasts, isolated from Vitis vinifera «Sakasly» and «Muscat d’Alexandrie» and protoplasts from embryogenic tissue of Vitis sp. «Seyval blanc» were incubated in the presence of PEG in a transformation solution containing the plasmid pBI426 which carries the β-glucuronidase (gus) and the neomycin phosphotransferase II (nptII) genes. The treated protoplasts were cultivated in CPW13 medium without kanamycin selection. 48h after the PEG transformation, transient expression of gus gene was detected histochemically and fluorimetrically in the protoplast cultures.

Authors

Rahma Jardak

Affiliation : Laboratoire d’Adaptation et d’Amélioration des Plantes, INRST, B.P. 95, 2050 Hammam-Lif, Tunisie

Ahmed Mliki

Affiliation : Centre de Biotechnologie de Borj cédria, Laboratoire de Physiologie Moléculaire des Plantes, B.P. 901, 2050 Hammam-Lif, Tunisia

Abdelwahed Ghorbel

ghorbelabdelwahed@yahoo.com

Affiliation : Institut of Biotechnology Laboratory of Grapevine Molecular Physiology BP 901 Hammam Lif 2050 Tunisia

Götz M. Reustle

Affiliation : Centrum Grüne Gentecknik (CGG), SLFA Neustadt, 67435 Neustadt/W, Germany

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