Original research articles

In vitro neoformation of grape chimeras (Vitis vinifera L.)

Abstract

Difference in grape sensitivity to Botrytis cinerea attacks between cultivars was explained by differences in the epidermic tissue of the fruit. Therefore, this work was conducted to create a grape periclinal chimera, whose fruits would combine the skin of a Botrytis tolerant cultivar with a pulp of an another cultivar admitted its good organoleptic quality and productivity. In a first time, graftings of two cultivars (Chardonnay and Pinot noir) were conducted in vitro on 5 different media supplemented with various plant growth regulators. Adventitious shoots were only observed on medium containing BAP and GA3 from a mixed callus structure after four weeks of darkness followed by a light/dark regime. In a second time, RAPD analysis, conducted on these plants, showed their chimerical characteristics.

Authors


Sandrine Verdisson

Affiliation : Laboratoire de Biologie et Physiologie Végétales; Laboratoire de Zoologie et des Sciences de l'Environnement (EA-2069), UFR Sciences, Université de Reims Champagne Ardenne, B.P.1039, 51687 Reims-02 (France)

s.verdisson@caramail.com

Fabienne Baillieul

Affiliation : Laboratoire de Biologie et Physiologie Végétales, UFR Sciences, Université de Reims Champagne Ardenne, B.P.1039, 51687 Reims-02 (France)


Jean-Claude Audran

Affiliation : Université de Reims Champagne-Ardenne, UFR Sciences, Laboratoire de Biologie et Physiologie Végétales, UPRES EA 2069 (URVVC), Reims, France

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