Original research articles

Effect of excessive nitrogen nutrition on free and conjugated polyamines content of leaves and berries of Vitis vinifera L. during their development

Abstract

Nitrogen is the most important element for higher plants, and plant productivity is to a large extend determined by nitrogen nutrition. Effects of nitrogen nutrition on levels of polyamines in leaves and grappes of two vines (Semillon and Sauvignon) are studied at different stages of their development (flowering, fruit set process, veraison and maturity). Two N-fertilizations are compared : N0 (0 kgN/ha/year) and N90 (90 kgN/ha/year). Analysis concerned the total level of nitrogen and polyamines (free and conjugates). Polyamines (putrescine, spermidine, spermine and diaminopropane) were derived by dansylation and analysed by CLHP. The results show that N-nutrition induces major changes in the total level of nitrogen and polyamines in various parts of plants. In all organs, excess of N-nutrition increases the total level of nitrogen. Concerning polyamines, these changes differe according to tissue and stage of development. In flowers and green berries, N-excess increases free polyamines whereas in berries during maturation N-excess increases total level of polyamines and particularly free and wall-bound polyamines. In opposite, in leaves, total level of polyamines increases under N-deficiency. These results reflect different rates of metabolism of polyamines between tissues. They suggest that in berries a restricted polyamines synthesis induced by nitrogen deficiency; whereas in leaves, N-deprivation can induce an accumulation of putrescine as do many others stress. The roles of polyamines in berries and in leaves are discussed.

Authors


Laurence Geny

Affiliation : Univ. Bordeaux, ISVV, EA 4577, USC 1366 INRA, Unité de recherche OEnologie, 210, Chemin de Leysotte 33882 Villenave d'Ornon, France

laurence.geny@oenologie.u-bordeaux2.fr

Michel Broquedis

Affiliation : Institut des sciences de la vigne et du vin (ISVV), Institut fédératif de recherche (IFR), Unité mixte de recherche (UMR) santé végétale, Institut national de la recherche agronomique (INRA), Centre de recherches de Bordeaux. B.P. 81, 33883 Villenave d'Ornon, France


Jean-Pierre Soyer

Affiliation : I.S.V.V., UMR Écophysiologie et Génomique Fonctionnelle de la Vigne, INRA, University of Bordeaux 2, University of Bordeaux 1, ENITA, Villenave d'Ornon, France; INRA, Domaine de la Grande Ferrade, BP 81, 33883 Villenave d'Ornon, France


Jacques Bouard

Affiliation : Institut de la Vigne de Bordeaux, Laboratoire des Sciences de la Vigne, Université Bordeaux 1, Avenue des Facultés, 33405 Talence cedex (France)

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